Fig. 2

Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses of DEGs responsive to PMMoV infection in pepper plants and validation of RNA-seq result. a GO analysis showing the GO terms in the categories of Cellular Component, Molecular Function and Biological Process (FDR < 0.05). b KEGG pathway enrichment analysis. The rich factor reflects the degree of enriched DGEs in given pathways. The number of enriched DGEs in various pathways is indicated by the size of the solid circle and the circle color represents the corrected q-value. c Twelve differentially expressed genes (DEGs) were selected based on the RNA-seq result and analyzed for their expressions in pepper after PMMoV infection through RT-qPCR using gene specific primers. The resulting RT-qPCR data were normalized using the expression level of C. annuum Ubiquitin-conjugating protein gene (CaUBI-3) and are presented as the means of fold change ± SD, relative to the Mock-inoculated plants. Asterisks indicate the statistically significant differences between the PMMoV- and Mock-inoculated pepper plants. (*p < 0.05, **p < 0.01)