Fig. 4

Effects of 3-MA and E64d on PMMoV RNA accumulation. a Total protein was isolated from PMMoV-infected N. benthamiana leaves treated with 3-MA, E64d or DMSO (mock). The protein samples were analyzed through Western blot assays using an anti-ATG8 or an anti-Rubisco antibody. The original data can be viewed from Additional file 11: Figure S4d-g. b Effect of 3-MA and E64d treatment on PMMoV infection. PMMoV-infected leaves treated with 3-MA, E64d or DMSO (mock) were harvested at 1 dpi, 3 dpi and 5 dpi, respectively. Total RNA was isolated from these leaf samples and analyzed through RT-qPCR. Values presented are the means of fold change ± SD, relative to that in the DMSO-treated control plants. Asterisks indicate statistical difference between treatments, determined by the Student’s t-test (*p < 0.05)