Fig. 7From: Genome-wide survey of soybean papain-like cysteine proteases and their expression analysis in root nodule symbiosisqPCR analysis of 28 selected GmPLCPs in nodule development. Soybean ELF1b and QACT were selected as reference genes to normalize the relative expression levels of each GmPLCP gene, and the expression level in 12dN was used as a starting point control to calculate the relative expression levels of other nodule samples. All qPCR reactions were repeated three times and the data are presented as the mean ± SDBack to article page