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Fig. 4 | BMC Plant Biology

Fig. 4

From: Prior exposure of Arabidopsis seedlings to mechanical stress heightens jasmonic acid-mediated defense against necrotrophic pathogens

Fig. 4

Regulation of defense gene expression in rosette leaves subject to MS and/or A. brassicicola infection. The 5th through 7th true leaves from control, infected, MS-treated and MS-infected 17-day old plants were inoculated with 5 μL of 3.5 × 104 spores/mL− 1 of A. brassicicola. Gene expression was quantified 5 dpi. A Gene expression of VSP1, OPR3, ERF3, PAD3, PDF1.2, and PR1 in WT. B Relative expression of VSP1, OPR3, ERF3, PAD3, PDF1.2, and PR1 in coi1–16 mutant compared to the WT. Relative gene expression levels were normalized to the β-ACTIN housekeeping gene. Error bars show standard error of the mean (n = 4 biological replicate). Letters are the statistical difference between treatments with Bonferroni test using a 2-way ANOVA, (P < 0.05). Statistical analysis in the coi1–16 mutant (B) is relative to the WT (A). The experiment was repeated twice, with similar expression patterns obtained

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