Table 1 List of qPCR primers
Primer name | Function |  | Primer sequence (5′-3′) | Locus |
|---|---|---|---|---|
PR1 | Induced by SA | F | TGCAGTGGGACGAGAGGGT | AT2G14610 |
R | CCACATGTTCACGGCGGAGA | |||
RBOHD | Response to ROS | F | GGTTTCAACGCCTTTTGGTA | AT5G47910 |
R | CGGTTTGATGCTTGATCTGA | |||
PDF1.2 | ET/JA response | F | GTTGCATGATCCATGTTTGG | At5g44420 |
R | CACCCTTATCTTCGCTGCTC | |||
ACTIN 2 | Reference gene | F | TGTCGCCATCCAAGCTGTTCTCTCC | AT3G18780 |
R | ACGTCCAGCAAGGTCAAGACGGAGG | |||
GSL6 | Callose deposition | F | CAAACCATGACGCTCCATAA | AT1G05570 |
R | GGTTCGTGGTATCACCCTTG | |||
PRX71 | Stem lignification | F | TTGTTGAACGGCAACGGAGTCACG | AT5G64120 |
R | AGCCGCTCGTGACACAGTCATTCT | |||
OPR3 | JA pathway | F | ACGTGGGAACCATCGGGCAACAAA | At2g06050 |
R | AGCAAGTTGTGGAAGCAGTTCACGC | |||
VSP1 | JA signalling | F | GGATCGAAGTTGACGCAAGTG | AT5G24780 |
R | CTCAACCAAATCAGCCCATTG | |||
PAD3 | Camalexin biosynthesis | F | TTCCTCTGTTTCCTCGTCCT | AT3G26830 |
R | ATGATGGGAAGCTTCTTTGG | |||
GST1 | Glutathione binding | F | TAATAAAAGTGGCGATGACC | AT1G02930 |
R | ACATTCAAATCAAACACTCG | |||
TCH3 | Calcium signalling | F | AGCCTTCCGCGTATTCGACAAGA | AT2G41100 |
R | CCGTCACCATCTGCATCCGC | |||
TCH4(XTH22) | Cell wall modification | F | TGTCTCCTTTGCCTTGTGTG | AT5G57560 |
R | GAAACTCCGCAGGAACAGTC | |||
ERF2 | Response to ET | F | TGAGGTTAATTCCGGTGAACC | AT3G23240 |
R | TCAACTTCCCGTTTTCAGACGA |