Fig. 7
From: GmbZIP1 negatively regulates ABA-induced inhibition of nodulation by targeting GmENOD40–1 in soybean
GmbZIP1 binds and represses GmENOD40–1 expression. a qRT-PCR analysis of GmENOD40–1 expression in transgenic empty or UBQ::GmbZIP1 overexpression roots at 14 DAI. Values are mean ± SD, p< 0.001. b Schematic representation of GmENOD40–1 locus. The GmENOD40–1 promoter was shown as a line and the ACGT motif (ACGTGT) was shown as grey box. c EMSA for DNA binding domain of GmbZIP1 fused with a His tag to oligo-DNAs containing ACGTGT. Biotin-labeled probe was incubated with GmbZIP1-His. Competition for binding was performed with different concentration of unbiotin-labeled probe. The experiments were repeated for three times, and the representative results were shown