Fig. 1

The effects of the proteasome inhibitor MG132 in Chlamydomonas. a The effect of 0, 5, 20, and 50 μM MG132 on levels of ubiquitinated proteins after 48 h of treatment were evaluated on SDS-PAGE electrophoresis. b Chlamydomonos were treated with 20 μM MG132 for 48 h, at which point viability and cell concentration were determined via flow cytometety. White and black columns represent viability and cell concentration, respectively, on the left and right axes. c The effect of 20 μM MG132 on cell concentration in Chlamydominas cultures were determined at different time points (0, 8, 24, and 48 h). d Cell volume was determined in cells treated with or without 20 μM MG132 at different time points. e The effect of 20 μM MG132 on cell volume; cells were grown for 48 h with or without MG132 and subsequently imaged using light microscopy. Shown are the means and standard errors of five replicate cultures, which are representative of two other experimental replicates. Asterisks represent a significant difference (p < 0.05) between treatments at each time point. L-ladder, kDA- kilodalton