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Fig. 5 | BMC Plant Biology

Fig. 5

From: Long-chain acyl-CoA synthetase 2 is involved in seed oil production in Brassica napus

Fig. 5

Identification of transgenic rapeseed lines. a Schematic diagram (not to scale) of a part the T-DNA region of BnLACS2-OE and BnLACS2-RNAi constructs used to transform canola. b PCR detection of positive BnLACS2-OE and BnLACS2-RNAi transgenic plants from a small number of resistant plants. c qRT-PCR analysis of BnLACS2 in different transgenic plant lines. The expression levels were quantified relative to the value obtained from WT plants. Error bars represent SD (n = 3). WT, non-transgenic canola; +, positive plasmid DNA; −, negative ddH2O, Lane 4–44, BnLACS2-OE transgenic plants, Lane 3–69, BnLACS2-RNAi transgenic plants

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