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Fig. 2 | BMC Plant Biology

Fig. 2

From: Transcriptome analysis reveals key genes involved in the regulation of nicotine biosynthesis at early time points after topping in tobacco (Nicotiana tabacum L.)

Fig. 2

Validation of RNA-Seq data by qRT-PCR. a Expression levels of 9 randomly-selected DEGs of the nicotine anabolic pathway as measured by qRT-PCR (the columns) and the corresponding expression trends measured by RNA-Seq (the lines). The error bars represent SDs (n = 3). Asterisks represent significantly different transcript levels between the topping treatment and control plants at the indicated times. (t-test; *, P < 0.05; **, P < 0.01; ***, P < 0.001). b Correlation analysis of fold-change data between qRT-PCR and RNA-Seq. Scatterplots are generated from the log2 expression ratios of qRT-PCR analyses (x-axis) and from RNA-Seq analyses (y-axis). Each scatter point depicts a time point at which significant differences in gene expression levels were found. The equation of the linear regression relationship and the associated correlation coefficient (R2) are provided

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BMC Plant Biology

ISSN: 1471-2229

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