From: Repair of sub-lethal freezing damage in leaves of Arabidopsis thaliana
Method | Non-acclimated | Cold acclimated | ||
---|---|---|---|---|
Samples frozen to | Samples frozen to | |||
non-lethal temperature (°C) | lethal temperature (°C) | non-lethal temperature (°C) | lethal temperature (°C) | |
Electrolyte leakage | − 3.0, − 3.5, − 4.5, − 5 | − 7 | − 8, − 8.5 | − 11 |
Chlorophyll fluorescence imaging | − 5, − 6, − 7 | − 8 | − 9, − 10, − 11 | − 12, − 13 |
Respiration | − 6, − 7 | − 8 | − 8, − 9 | − 12 |
Sugar content | − 4.5, − 5, − 6, − 7 | − 8 | − 8, − 8.5, − 9, − 10, − 11 | − 12, − 13 |
Proline content | − 4.5, − 5, − 6, − 7 | − 8 | − 8, − 8.5, − 9, − 10, − 11 | − 12, − 13 |
Gene expression analysis (qRT-PCR) | − 5, − 6, − 7 | − 8 | − 9, − 10, − 11, | − 12, − 13 |