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Table 1 Treatment temperatures of leaves for different measurements. Freezing temperatures were chosen according to previously reported LT50 values of − 5.34 °C NA and − 9.68 °C ACC [28]

From: Repair of sub-lethal freezing damage in leaves of Arabidopsis thaliana

Method

Non-acclimated

Cold acclimated

Samples frozen to

Samples frozen to

non-lethal temperature (°C)

lethal temperature (°C)

non-lethal temperature (°C)

lethal temperature (°C)

Electrolyte leakage

− 3.0, − 3.5, − 4.5, − 5

− 7

− 8, − 8.5

− 11

Chlorophyll fluorescence imaging

− 5, − 6, − 7

− 8

− 9, − 10, − 11

− 12, − 13

Respiration

− 6, − 7

− 8

− 8, − 9

− 12

Sugar content

− 4.5, − 5, − 6, − 7

− 8

− 8, − 8.5, − 9, − 10, − 11

− 12, − 13

Proline content

− 4.5, − 5, − 6, − 7

− 8

− 8, − 8.5, − 9, − 10, − 11

− 12, − 13

Gene expression analysis (qRT-PCR)

− 5, − 6, − 7

− 8

− 9, − 10, − 11,

− 12, − 13