Fig. 2

Subcellular localization of TaSTP13 in plants. a Transient expression of free GFP (control) and TaSTP13-GFP under control of the 35S promoter in wheat leaf protoplasts. b GFP and TaSTP13-GFP fusion proteins expressed in N. benthamiana by transient agro-infiltration assays. The All signals were monitored using confocal microscopy. FM4–64 was used to stain the plasma membrane. GFP fluorescence is in green; red fluorescence is from FM4–64 to label the plasma membrane or auto-fluorescence from chloroplasts. Merged images are depicted here. Bright field images show the equivalent field observed under white light. Comparable expression and localization patterns were observed in three independent biological replicates