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Fig. 5 | BMC Plant Biology

Fig. 5

From: TaSTP13 contributes to wheat susceptibility to stripe rust possibly by increasing cytoplasmic hexose concentration

Fig. 5

Histological observation of fungal growth and host response in wheat infected with BSMV:GFP and recombinant BSMV after inoculation with the Pst pathotype, CYR31. Growth of CYR31 in wheat leaves inoculated with BSMV:GFP, BSMV:TaSTP13-as1, and BSMV:TaSTP13-as2 at 48 hpi (a–c), 72 hpi (d–f), and 120 hpi (g–i) was observed under a fluorescence microscope. j The numbers of branches of infection hyphae (HB), haustorial mother cells (HMC) and haustoria (H) per infection unit were recorded at 48 hpi. k The width of infection hyphae (IH) in TaSTP13-silenced plants at 48 hpi with the Pst pathogtype CYR31. The width of IH was approximately perpendicular to substomatal vesicle (SV) and the apex of IH. l The ratio of aberrant swelled hypha structure in wheat inoculated with BMSV constructs at 48 hpi. The aberrant swelled hypha structure was defined as hypha, of which, width was larger than the mean width of IH in BSMV:GFP-infected wheat. TaSTP13-silenced plants had a significantly reduced infection unit area at 72 hpi (m) and 120 hpi (n). Values represent the mean ± SD of three independent samples with 50 infection sites each time. Asterisks indicate a significant difference (**P < 0.01) from BSMV: GFP inoculated plants. Differences were assessed using a one-way ANOVA and Student’s t-test

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