Skip to main content
Fig. 10 | BMC Plant Biology

Fig. 10

From: Zn/Cd status-dependent accumulation of Zn and Cd in root parts in tobacco is accompanied by specific expression of ZIP genes

Fig. 10

Complementation by NtZIP5B cDNA of yeast mutants defective in metal uptake on selective media. Yeast cells: DY1457, Δzrt1zrt2 (defective in Zn uptake) were transformed with the empty vector pAG426 as a control (e.v.), or with the vector carrying NtZIP5B with the stop codon pAG426-ZIP5B (NtZIP5B). Yeast cultures were adjusted to an OD600 of 0.2, and 3 μl of serial dilutions (from left to right in each panel) was spotted on SC-URA medium containing 2% (w/v) galactose solidified with 2% agar, supplemented with CdCl2 (a); EGTA (b), or ZnCl2 (c). The plates were incubated for 3–6 d at 30 °C. The images are representative of three independent experiments taken after 3 days of growth

Back to article page