Fig. 4From: Zn/Cd status-dependent accumulation of Zn and Cd in root parts in tobacco is accompanied by specific expression of ZIP genesExpression of tobacco ZIP genes in the apical, middle and basal parts of roots from plants grown under various Zn and Cd concentrations. 3.5-week old plants grown in the quarter-strength Knop’s medium were exposed to the control medium supplemented with pairwise combinations of Zn (0; 1 μM) and Cd (0; 0.25; 1 μM) for 17 days. ZIP genes preferentially expressed in the apical root part (a): NtZIP2 (a1), NtZIP5A (a2), NtZIP5B (a3), NtIRT1 (a4), NtIRT1-like (a5); in the basal root part (b): NtZIP1-like (b1), NtZIP8 (b2); equally expressed in tested three root parts (c): NtZIP4A (c1), NtZIP4B (c2); mixed expression pattern for tested three root parts (d): NtZIP5-like (d1), NtZIP11 (d2). Gene expression was normalized to the PP2A level. Values correspond to arithmetic means ±SD (n = 3); those with the ratio greater than 2 are considered significantly differentBack to article page