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Table 2 Summary of the identification of TaALPs in wheat varieties Spitfire and Mace

From: Characterising avenin-like proteins (ALPs) from albumin/globulin fraction of wheat grains by RP-HPLC, SDS-PAGE, and MS/MS peptides sequencing

ALPs

AAs

Cysteine residues

Main Peak of HPLC

Retention time (Min)

MW1 a (kDa)

MW2 b (kDa)

MW2 c (Da)

MW2 d (Da)

C-terminal TaALP-by-7DS

152

11

Mace-8

17.03

17.39

18.54

–

–

C-terminal TaALP-by-4AL

152

11

Mace-8

17.03

17.44

18.60

–

–

TaALP-ay-7DS

154

14

Mace-11

20.24

16.96

18.42

9133.37

10,070.63

TaALP-ay-4AL

153

14

Mace-17

22.94

17.01

18.47

9198.44

10,135.7

TaALP-ax-4AL

162

14

Mace-20

24.72

18.01

19.47

10,198.54

11,135.8

TaALP-ax-7AS

156

14

Mace-26

28.75

17.29

18.75

9579.93

10,517.19

TaALP-ax-7DS

149

14

Mace-29

30.94

16.44

17.90

9914.25

10,851.51

TaALP-by-7DS

261

19

Mace-8-12

17.03–21.02

29.97

31.95

25,439.88

27,406.12

TaALP-by-4AL

261

19

Mace-8-12

17.03–21.03

29.87

31.84

24,533.1

26,095.2

TaALP-by-7AS

261

19

Mace-8-12

17.03–21.04

29.69

31.67

25,347.9

27,014.14

TaALP-bx-7DS

266

18

Mace-13-23

21.61–26.34

30.59

32.46

–

–

TaALP-bx-4AL

267

19

Mace-13-24

21.61–26.35

30.88

32.86

–

–

C-terminal TaALP-by-7DS

152

11

Spitfire-8

17.46

17.39

18.54

–

–

C-terminal TaALP-by-4AL

152

11

Spitfire-8

17.46

17.44

18.60

–

–

TaALP-ay-7DS

154

14

Spitfire-11

20.26

16.96

18.42

9133.37

10,070.63

TaALP-ay-4AL

153

14

Spitfire-15

23.14

17.01

18.47

9198.44

10,135.7

TaALP-ax-4AL

162

14

Spitfire-21

27.67

17.74

19.20

10,157.53

11,094.79

TaALP-ax-7AS

156

14

Spitfire-23

29.37

17.29

18.75

9579.93

10,517.19

TaALP-ax-7DS

149

14

Spitfire-25

31.11

16.44

17.90

9914.25

10,851.51

TaALP-by-7DS

261

19

Spitfire-8

17.46

29.97

31.95

25,439.88

27,406.12

TaALP-by-4AL

261

19

Spitfire-8

17.46

29.87

31.84

24,533.1

26,095.2

TaALP-bx-7DS

266

18

Spitfire-13-19

21.97–26.60

30.59

32.46

–

–

TaALP-bx-4AL

267

19

Spitfire-13-19

21.97–26.60

30.88

32.86

–

–

TaALP-bx-7AS

266

18

Spitfire-13-19

21.97–26.60

30.52

32.39

24,853.38

26,519.62

  1. aCalculated molecular weight of ALPs; b Calculated molecular weight of ALPs after molecule alkylation; c Calculated molecular weight of cleaved ALPs; dCalculated molecular weight of cleaved ALPs after molecule alkylation. Note: The theory was that each cysteine residue would combine with one 4-vp molecule and the molecular mass would increase 104.14 Da (the 4-vp molecular mass minus the mass of one hydrogen ion). The cleavage of ALP occurred at the predicted myristoylation cleavage sites
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BMC Plant Biology

ISSN: 1471-2229

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