Skip to main content
Fig. 4 | BMC Plant Biology

Fig. 4

From: Transcriptome analysis reveals insight into molecular hydrogen-induced cadmium tolerance in alfalfa: the prominent role of sulfur and (homo)glutathione metabolism

Fig. 4

Effects of H2, L-buthionine-S,R-sulfoximine (BSO), and reduced glutathione (GSH) on the Cd-induced inhibition of alfalfa seedling growth and fresh weight (a), root elongation (b), and thiobarbituric acid-reactive substance (TBARS) content (c) in alfalfa roots. Five-day-old seedlings were pretreated with or without HRW, 500 μM BSO, and 1 mM GSH individually or in combination for 12 h followed by another 72 h (a and b) and 24 h (c) treatment with 100 μM CdCl2. Data in the top and bottom of part (a) indicate the fresh weight of 30 seedlings above ground and underground, respectively. Bar = 2 cm. Values are the mean ± SE of three independent experiments with at least three replicates for each. Bars with different letters indicate significant differences (P < 0.05) according to Duncan’s multiple range test

Back to article page