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Fig. 7 | BMC Plant Biology

Fig. 7

From: Transcriptome analysis reveals insight into molecular hydrogen-induced cadmium tolerance in alfalfa: the prominent role of sulfur and (homo)glutathione metabolism

Fig. 7

Content of Cd and the transcripts of ABC transporter genes in alfalfa seedling roots. Five-day-old seedlings were pretreated with or without HRW for 12 h followed by another 12 h (b-f) or 72 h (a) treatment with 100 μM CdCl2. The Cd content in alfalfa seedling roots was determined after 72 h of Cd exposure (a). Relative expression of the ABC transporter gene homologues of Mtr_1g086080 (b), Mtr_4g077930 (c), Mtr_4g124040 (d), Mtr_6g008800 (e), and Mtr_6g008820 (f) was detected by RT-qPCR. The sample without chemicals was the control (Con). Expression levels of corresponding genes are presented relative to the control samples and were normalized to the expression of two internal reference genes in each sample. Values are the mean ± SE of three independent experiments with at least three replicates for each. Bars with different letters indicate significant differences (P < 0.05) according to Duncan’s multiple range test

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