Reducing the size of an alien segment carrying leaf rust and stripe rust resistance in wheat

BMC Plant Biology

Table 1 PCR markers for mapping the Ae. sharonensis alien segment

No.	Marker name	Primer (5′-3′)		Annealing Temp^a (C°)	Marker size (bp)
No.	Marker name	Forward	Reverse	Annealing Temp^a (C°)	Marker size (bp)
1	1C	GGCCAGTGCAATAAACT	ATTTGTAGTAAGAGTGCG	56	214
2	2S1	AAAAGAAAGTTGGCCCCG	CGGCATGATTAAAACATGAGGCAA	61	128
3	2S2	TCATCGATGGGATCGAC	ATGTCCACCTGTCCCAAG	62	209
4	3S1	ATCCTATCGCTCAACATG	ACAGTTAGCTTGGCTTCA	56	147
5	3S3	TGATGGATTGGATTAAAAACTTG	GTCCTTTTCTCCATCTTCATC	56	153
6	3S4	GCTGCGTAAAATTAAGCA	CTTTTAGTCAATTCTTGGTC	56	180
7	4C	CTCAATCATTTCCGTCTAC	CTACGCAACAAGGAAAACA	57	191
8	2-3HS2	CAAACACCACAACAGCTATG	CAGCCCGAAGGAAAACAAT	61	154
9	2-3HS3	CAATTGGCATAAGAGCCTT	CTCGACGATGATGAAGAC	61	144
10	PSR2120	TTAACGCCAGGGCATACTC	CTGCAGGAGGCGCTGGA	58	232
11	Zyg_1Sh_1	CAAACACCACAACAGCTATG	CAGCCCGAAGGAAAACAAT	61	154
12	Zyg_2G_2	CAATTGGCATAAGAGCCTG	CATAGCCATCACCACCTTG	58	219
13	3614_1	ATCGCAAGGTGTTGTCCATT	GGCAGCTGGAAGATCAAGTC	56	165

In all markers, which are based on SNPs, the polymorphisms are in bold. Markers 1–7 were used to characterize the length of the segment. Markers 8–9 were used for detection of the critical area for the resistance. Marker 10 was used for selection of ph1b/ph1b lines. Markers 11–12 were used simultaneously for selection of plants homozygous for the segment (plants in which marker 11 was present and marker 12 was absent were selected). Marker 13 was used for the assessment of recombinant area at the distal long arm telomere area. Temperature cycling consisted of 95 °C for 5 min, followed by 32 cycles of 95 °C for 30 sec, Annealing Temperature (see table for details) for 30 sec, 72 °C for 30 sec with a final extension step at 72 °C for 5 min
^aAnnealing temperature varied depending on the Taq polymerase used

ISSN: 1471-2229