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Fig. 1 | BMC Plant Biology

Fig. 1

From: Crystal structure of DRIK1, a stress-responsive receptor-like pseudokinase, reveals the molecular basis for the absence of ATP binding

Fig. 1

Maize DRIK1 is a conserved RLK expressed in roots and leaves. a Phylogenetic analysis of ZmDRIK1. Amino acid sequences of representative proteins of the subfamily LRR-VI-2 from several species were aligned, and a phylogenic tree was constructed using the out-groups AtSERK1, AtBRI1, AtCLV1 (catalytically active kinases), and AtBSK8 (catalytically inactive kinase). b Intron/exon structure of the ZmDRIK1 gene compared with closely related genes from maize, sorghum, rice and Arabidopsis. Exons are colored arrows, and introns are black lines. The transmembrane domain (green dotted line) and the kinase domain (red dotted line) corresponding regions are shown. Numbers indicate nucleotide positions along the gene sequence. cZmDRIK1 relative mRNA content in roots and leaves at 3 and 15 days after sowing (DAS), as determined by qRT-PCR. β-tubulin was used to normalize samples. At alpha 0.05 level, the relative mRNA level of ZmDRIK1 in leaves at 15 DAS was significantly greater than that level in roots, as well as greater than the mRNA level in leaves and roots from younger seedling (3 DAS). The p-values of Student’s t test between tissues or age from seedlings, are indicated above the comparative lines in the figure. Error bars indicate standard deviation between three biological replicates

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