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Fig. 2 | BMC Plant Biology

Fig. 2

From: Potato virus X-mediated constitutive expression of Plutella xylostella PxSDF2L1 gene in Nicotiana benthamiana confers resistance to Phytophthora parasitica var. nicotianae

Fig. 2

PVX-mediated recombinant gene expression in N. benthamiana. a Schematic representation of PVX.GFP (control) and PVX.SDF2L1 binary constructs carrying the recombinant genes gfp and PxSDF2L1, respectively. In the scheme: LB and RB, left and right T-DNA border sequences; p35S, 35S promoter of cauliflower mosaic virus; RdRp, PVX RNA-dependent RNA polymerase gene; 25 K, 8 K, and 12 K, PVX movement protein genes; pCP, subgenomic promoter sequence (duplicated); CP, PVX coat protein gene; Nos, nopaline synthase transcriptional terminator. b RT-PCR reaction of PxSDF2L1 in RNA samples from the root of plants agroinfected with recombinant PVX vectors, 21 d.p.ai. Lane 1: PVX.GFP plant (negative control); Lanes 2–7: PVX.PxSDF2L1 plants. M, 1 kb DNA ladder

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BMC Plant Biology

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