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Fig. 4 | BMC Plant Biology

Fig. 4

From: Cloning and overexpression of PeWRKY31 from Populus × euramericana enhances salt and biological tolerance in transgenic Nicotiana

Fig. 4

Generation of PeWRKY31-overexpressing transgenic tobacco and PCR analysis of the ten transformed tobacco lines. a: Leaves used for Agrobacterium infection. b: Screening for resistant vegetative propagules. c: Rooted plantlet. d: Transplanted plantlet. e: nptII gene fragment (~ 500 bp) amplified from transgenic tobacco. f: Portion of PeWRKY31 gene (~ 400 bp) amplified from transgenic tobacco. Lane M, DL2000 DNA marker of US EVERBRIGHT; other lanes, test samples

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