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Table 3 Quantitative and qualitative description of the traces (of ovules or seeds) and the seeds extracted from the berries inspected at veraison for the investigated genotypes

From: Somatic variants for seed and fruit set in grapevine

Accession

Berry size

N of berries

Berry diameter range (mm)

N of traces

N of seeds

N of potentially vital seedsa

Traces per berry

Seeds per berry

Observations

Corinto Nero

Small

10

8.2 - 10.2

16

0

-

1.6

0

Very small traces. They were very soft, impossible to dissect because they fell apart. Traces completely absent in two berries out of ten

Corinto Nero

Large

10

13.0 - 16.3

13

10

10

1.3

1.0

Despite all seeds were potentially vital, when dissected, some of them presented a degenerated endosperm. An embryo was observed in some seeds with a well-developed endosperm

Sangiovese

Unique

10

12.2 - 14.2

0

24

24

0

2.4

 

Aspirant

Small

10

NA

23

0

-

2.3

0

Some traces were completely soft, while others showed a partially developed sclerenchyma

Aspirant

Large

10

NA

29

0

-

2.9

0

In general, traces extracted from the biggest berries were more developed than those from smaller berries

Liseiret

Small

10

NA

14

18

12

1.4

1.8

 

Liseiret

Large

10

NA

10

33

22

1.0

3.3

 

Moscato Bianco mutant

Small

10

7.0 - 8.4

0

0

-

0

0

Berries with no traces at all

Moscato Bianco mutant

Large

2

13.8 - 17.0

0

4

4

0

2.0

Seeds presented a well-developed endosperm

Moscato Bianco

Unique

10

13.0 - 16.6

0

19

19

0

1.9

 

Termarina Rosa

Small

10

7.0 - 8.2

32

0

-

3.2

0

Very small and soft traces, impossible to dissect because they were destroyed due to the reduced size and fragility

Termarina Rosa

Large

10

8.2 - 9.9

24

0

-

2.4

0

Very small and soft traces, impossible to dissect because they were destroyed due to the reduced size and fragility

Termarone

Unique

10

14.0 - 16.2

0

29

28

0

2.9

 

Chasselas apyrène

Small

10

11.2 - 14.1

27

0

-

2.7

0

Soft traces, heterogeneous in size. Some of them, despite being soft and with no well distinguishable structure inside, had the appearance of a seed on a reduced scale

Chasselas apyrène

Large

10

14.5 - 16.8

8

15

4

0.8

1.5

The potentially vital seeds contained a well-developed endosperm. The seeds that floated where hollow inside, when dissected only seed coat and sclerenchyma structures were observed, while endosperm was usually not present (nor embryo) or was partly degenerated

Sultanina

Unique

10

11.2 - 14.2

29

0

-

2.9

0

Soft traces, heterogeneous in size. A seed shape (pear shape) was observed for the biggest ones, which could also be longitudinally or transversally dissected. No sclerenchyma was formed and inside a very thin white tissue with an aqueous or gel-like consistency could be distinguished

Corinthe Noir

Small

11

7.5 - 9.9

19

0

-

1.7

0

Traces were tiny and soft, it was impossible to dissect them because they were destroyed due to their reduced size and fragility

Corinthe Noir

Large

3

12.5 - 17.3

5

4

1

1.7

1.3

Traces were similar to those of the smaller berries. Almost all inspected seeds were empty. One out of the four seeds presented an endosperm, but no embryo was observed

  1. Abbreviations: N number, NA not analyzed
  2. aBased on the floatation test: seeds that sank into water were considered potentially vital