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Table 6 Missense single nucleotide polymorphisms between Sangiovese (SG) and Corinto Nero (CN) that were detected in the RNA-Seq dataset described by [52] and confirmed by Sanger sequencing. For each variant allele (highlighted in bold), somatic line specificity in comparison with the PN40024 (PN) reference genome, position, locus affected, locus functional annotation, genotype of each cultivar, and predicted effect in the protein sequence and function are shown.

From: Somatic variants for seed and fruit set in grapevine

Variant type

Variant position

Gene ID

Functional annotation

Genotype

Variant allele effect on protein sequence

Variant amino acid effect on protein function

PROVEAN scorea

PN

SG

CN

SG specific

Chr2:2,837,618

VIT_02s0025g03330

H(+)-ATPase 4 AHA4

A:A

A:G

A:A

Y274C

Deleterious

-7.74

CN specific

Chr6:4,776,574

VIT_06s0004g03800

DNA-binding protein

C:C

C:C

C:T

T1383M

Neutral

-1.06

CN specific

Chr11:2,940,013

VIT_11s0016g03590

Transducing protein

A:A

A:A

A:G

I1114V

Neutral

0.48

CN specific

Chr11:5,363,589

VIT_11s0016g05820

CCR4-NOT transcription complex subunit 10

G:G

G:G

A:G

V317M

Neutral

-0.76

SG specific

Chr14: 23,114,120

VIT_14s0083g00910

Auxin-independent growth promoter

C:C

C:T

C:C

R536C

Deleterious

-2.71

  1. aVariants with a PROVEAN score equal to or below -2.5 were considered "deleterious," variants with a score above -2.5 were considered "neutral"