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Fig. 6 | BMC Plant Biology

Fig. 6

From: Genome-wide analysis of the apple CaCA superfamily reveals that MdCAX proteins are involved in the abiotic stress response as calcium transporters

Fig. 6

Characterisation of calcium transport activity in apple CAX family proteins. a Phenotype of yeast cells treated with different concentrations of calcium ions. The label above the picture shows the concentration of CaCl2 uses in the calcium ion treatment. b Bright-field and fluorescence microscopy of yeast cells labelled with the calcium-sensitive fluorescent probe Fluo4-AM. The scale bar represents 5 μm. MdCAX3L-1 to MdCAX3L-4 represents the transformation of K667 yeast strains with corresponding full-length MdCAX genes, and MdCAX3L-1(−N32) represents the transformation with the truncated MdCAX3L-1 gene, with 32 amino acids of its N terminus removed. K667 or K667 transformed with the empty vector pDR196 were used as controls. c Average grey values of green fluorescence in vacuoles of K667 yeast cells. The gray values of green fluorescence in the vacuoles of ten yeast cells expressing a specified MdCAX gene were used to calculate the average grey value of green fluorescence. Bars labelled with different letters indicate signigicant differences at P < 0.05 based on one-way ANOVA and Duncan’s tests (SPSS software, version 26)

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