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Fig. 3 | BMC Plant Biology

Fig. 3

From: Physical mapping of repetitive oligonucleotides facilitates the establishment of a genome map-based karyotype to identify chromosomal variations in peanut

Fig. 3

Sequential fluorescence in situ hybridization/genomic in situ hybridization (FISH/GISH) karyotype and chromosome plots of the cultivar Tifrunner. a FISH using Multiplex #3 (green) and Multiplex #4 (red); b GISH using genomic DNA of A. duranensis (green) and A. ipaensis (red); c FISH using 45S rDNA (red) and 5S rDNA (green) as the probes; d Karyotypes corresponding to chromosomes in the sequencing map of Tifrunner; SSON indicates the FISH karyotype using Multiplex #3 (green) and Multiplex #4 (red); GISH indicates the FISH karyotype using total genomic DNAs of A. duranensis (green) and A. ipaensis (red); 45S/5S indicates the FISH karyotype using 45S rDNA (red) and 5S rDNA (green); e Idiogram karyotype of Tifrunner; f chromosome plots of Tifrunner; blue arrows in (e) and (f) indicate the non-correspondent signals of oligos Ipa-1162 and Ipa-1137 between the karyotype and chromosome plots; green arrows in (e) and (f) indicate the non-correspondent signals of oligos TIF-439, TIF-185-1, TIF-134-3 and TIF-165-3 between the karyotype and chromosome plots; and red arrows in (e) and (f) indicate the non-correspondent signals of oligos DP-5 between the karyotype and chromosome plots. Scale bar: 10 μm

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