Fig. 5

Chromosome variants detected in the radiation-induced M₁ plant 161-1a of the peanut cultivar Silihong (SLH) after sequential fluorescence in situ hybridization/genomic in situ hybridization (FISH/GISH). a–d Results of sequential FISH/GISH in SLH (a–b) and M₁ plant 161-1a (c–d), using Multiplex #3 (green) and Multiplex #4 (red) (a and c); A. duranensis genomic DNA (b and d; green); A. ipaensis genomic DNA (b and d; red). e Karyotypes of SLH and 161-1a. f–g Sequential FISH/GISH using probe Libraries L1A-1 (green) and L3A-1 (red). h Translocated chromosomes in 161-1a. SSON indicates the FISH karyotype using Multiplex #3 (green) and Multiplex #4 (red); GISH indicates the FISH karyotype using total genomic DNAs of A. duranensis (green) and A. ipaensis (red). Scale bar: 10 μm