Fig. 4

Effect of salt stress on the expression of ABI4 and VTC2. The expressions of ABI4 in Col-0 a, VTC2 in Col-0 b, VTC2 in abi4–103 c, VTC2 in ABI4-OEM1 d seedlings under 200 mM NaCl were analyzed by qPCR. After normalizing to the internal control ACTIN, the transcript levels of ABI4 and VTC2 at the indicated treatment times were compared with that at 0 h, which was normalized as “1”. Error bars represent the SD from three biological replicates. Statistically significant differences are indicated by star symbol (P < 0.05, Mann-Whitney U test). e Comparison of VTC2 protein levels in Col-0 and abi4 seedlings. The numbers under the bands indicate the relative light intensity of bands, the detected band in pVTC2::VTC2-GFP/Col-0 without NaCl treatment was normalized as “1”. Total proteins were extracted from pVTC2::VTC2-GFP/Col-0 and pVTC2::VTC2-GFP/abi4–103 seedlings under 100 mM NaCl treatment for 24 h and detected using anti-GFP antibodies. The detection of ACTIN was used as loading control