Fig. 3

Effects of ABA on cotyledon greening of the aitr mutants. a Cotyledon greening of the Col wild type and the aitr mutants in response to ABA treatment. Seeds of the Col wild type, the aitr256 triple, aitr1256 quadruple, aitr23456 quintuple and aitr123456 sextuple mutants were sterilized and sown on 1/2 MS plates in the presence or absence of 2.5 µM ABA. The plates were transferred, after kept at 4 °C in darkness for 2 days, to a growth room. Pictures were taken 17 days after the transfer. b Quantitative assays of cotyledon greening of the Col wild type and the aitr mutants in response to ABA. Green seedlings were scored 17 days after the transfer, and percentage of green seedlings was calculated. The experiments were repeated three times, and similar results were obtained. Data represent the mean ± SD of three replicates