Fig. 5

Interactions of GhFT and GhFD with each GhGRF protein. a Phylogenetic analysis of 31 GhGRF proteins. Red pentagram represent the selected GhGRF proteins used to validate interaction with GhFT. b Y2H experiment between GhFT and each GhGRF protein. c Y2H experiment between GhFD and each GhGRF protein. Plasmids transformed into AH109 yeast strains were screened on the -LT and -LTHA medium. -LT and -LTHA medium represent SD/−Trp-Leu and SD/−Trp-Leu-His-Ade medium. d BiFC verification of interactions between GhFT and each GhGRF protein. e BiFC verification of interactions between GhFD and each GhGRF protein. Constructs of cYFP-GhGRFs and nYFP-GhFD were co-transformed into N. benthamiana leaf epidermal cells