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Fig. 7 | BMC Plant Biology

Fig. 7

From: A pattern-triggered immunity-related phenolic, acetosyringone, boosts rapid inhibition of a diverse set of plant pathogenic bacteria

Fig. 7

Membrane depolarization and permeability testing of bacteria after treatment with oxidized AS as a function of bacterial concentration. Dilution series of bacteria from 5 × 107 to 0.625 × 107 CFU/ml of P. syringae pv. tabaci and A. tumefaciens C58C1 bacteria was added to reaction mixtures containing 50 μM AS, 50 μM H2O2, and 0.72 U/ml POX. Untreated bacteria were used as negative, and heat killed bacteria as positive controls. Bacterial membrane polarity was tested with the voltage sensitive DIBAC4(3) stain, and membrane permeability with SYTOX Green stain, both added 3 h after treatment of bacteria. Error bars indicate standard deviations. Asterisks indicate significant difference from corresponding controls according to student’s T-test (*p < 0.1; **p < 0.05). Abbreviations: BACT: bacterium, AS: acetosyringone, HK: heat-killed, POX: horseradish peroxidase, AU: arbitrary unit

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