Fig. 6
From: Genome-wide identification, characterization and expression analysis of BES1 gene family in tomato
Transactivation activity analysis of SlBES1 proteins. a Transactivation activity analysis of SlBES1 proteins in yeast. The pGBKT7-SlBES1 fusion vectors were transformed into Y2H Gold yeast cells. The transformants were cultivated on SD/−Trp medium and screened by X-α-Gal and Aureobasidin A (AbA). b Transcriptional repression activity analysis of SlBES1.2 and SlBES1.8 by dual-luciferase assay. The vector construction of effector and reporters are shown above. VP16 and pBD alone were used as positive and negative control respectively. The ratio of LUC to REN indicates the trans-repression ability of SlBES1.2 and SlBES1.8. Value of each column represents the mean ± SE of six biological replicates. *** refer to significant differences with p < 0.001