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Fig. 2 | BMC Plant Biology

Fig. 2

From: RUS6, a DUF647-containing protein, is essential for early embryonic development in Arabidopsis thaliana

Fig. 2

Identification and molecular characterization of heterozygous T-DNA lines with RUS6 gene interruptions. a and b T-DNA insertions in RUS6 gene (AT5G49820) were detected in two T-DNA lines: GK027G06 and emb1879. Filled boxes indicate RUS6 exons, greyed-out portions indicate deletions. Positions for the start codon (ATG) and the stop codon (TAA) of RUS6 are indicated. Locations of T-DNA insertion in the RUS6 gene and PCR primers used to detect either the T-DNA insertion (RUS6-1936F and TJLB155 for GK-27G06 line; Garlic LB3 and RUS6-RTR for emb1879 line) or a wild-type RUS6 fragment (RUS6-1936F and RUS6-CDS2-R for the GK-027G06 line; RUS6-P3-F and RUS6-R1 for the emb1879 line) are indicated. Gel images of PCR detection of either the T-DNA (T-DNA Detection) or the wild-type RUS6 fragments (RUS6 Detection) are shown (right panels). For both GK-27G06 and emb1879, rus6 mutant alleles (rus6-1 and rus6-2) can only be maintained as a heterozygote (rus6/ +). 1 kb: 1 kb marker. kb: kilobase. c Segregation of sulfadiazine-resistance marker in offspring of self-fertilized rus6-1/ + . 10-days-old seedlings were grown on MS plates containing sulfadiazine. Healthy green seedlings are individuals carrying the sulfadiazine-resistance maker and unhealthy brown seedlings (indicated by an arrow) are WT seedlings. d and e Heterozygous mutants (rus6-1/ + and rus6-2/ +) are indistinguishable from the wild-type (WT) at various stages of development. d Representative images of 7-days-old seedlings (rus6-1/ + , rus6-2/ + , WT) grown on MS plates are shown (bar = 0.5 cm). e Representative mature plants with inflorescences (42-days-old) are shown (bar = 2 cm)

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