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Fig. 8 | BMC Plant Biology

Fig. 8

From: Genome-wide identification and expression analysis of aquaporin family in Canavalia rosea and their roles in the adaptation to saline-alkaline soils and drought stress

Fig. 8

The spot assays for stress tolerance confirmation of the CrPIP1;5 expression in yeast. a Map of the CrPIP1;5-pYES-DEST52 construct. b High osmotic stress and salt tolerance confirmation in yeast. The growth state of yeast cells (WT, BY4741) transformed with CrPIP1;5-pYES-DEST52 and empty vector pYES2 with or without PEG8000, sorbitol, or NaCl on SDG-Ura plates. The concentration of these stress factors for each treatment was labeled on the left. c H2O2 oxidative stress tolerance confirmation in yeast. The growth state of yeast cells (WT and H2O2-sensitive mutant strain skn7Δ) transformed with CrPIP1;5-pYES-DEST52 and empty vector pYES2 with or without H2O2 on SDG-Ura plates. The concentration of H2O2 for each treatment was labeled on the left

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