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Fig. 4 | BMC Plant Biology

Fig. 4

From: Anther dehiscence is regulated by gibberellic acid in yellow lupine (Lupinus luteus L.)

Fig. 4

Gibberellic acid (GA3) immunolocalization in selected stages (1–4) of late anther development (LAD) in yellow lupine. Green fluorescence corresponds to GA3 accumulation, and blue fluorescence indicates cell nuclei stained with DAPI. Subfigures A1/B1 are an enlargement of subfigures A/B, respectively, in the places marked with the red squares. The red arrows indicate GA3 signal in the selected magnified cells. The yellow squares marked with a dashed line in subfigures B and B′ indicate the same area of cells. Autofluorescence of the cell walls and pollen grains was visible. VB – vascular bundle, P – pollen grain, E – epidermis, En – endothecium, T – tapetum, C – connective, Se – septum, St – stomium. Scale bars: 25 μm (A, B, B′, C, D), 10 μm (A1) and 5 μm (B1)

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