Fig. 4From: Predominantly symplastic phloem unloading of photosynthates maintains efficient starch accumulation in the cassava storage roots (Manihot esculenta Crantz)The expression and enzymatic activities of genes involved in post phloem unloading in the storage root of cassava. a, b Relative expression difference of CWI and SuSy measured by Q–PCR. c Heat map comparisons of differential gene expression of CWIs, SuSys and SUTs. d The expression profiling of SUTs (SUT1, SUT2 and SUT4) in leaves and storage roots measured by Q–PCR. e The expressed protein levels of SuSy, CWI and SAI assessed by immunoblotting. f Enzymatic activities of SuSy (triangles), CWI (squares) and SAI (circles). The column chart in a, b, d and the curve chart in f were performed by Micro software Excel 2016. All of them were with cropped edges and adjusted position using Adobe Photoshop CS6.0 software. E, Early stage of storage root; M, Middle stage of storage root; L, Late stage of storage root; PFR, Primary fibrous root and SFR, Secondary fibrous root; a, b and c denote significantly different levels at P < 0.05Back to article page