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Fig. 2 | BMC Plant Biology

Fig. 2

From: The core autophagy machinery is not required for chloroplast singlet oxygen-mediated cell death in the Arabidopsis thaliana plastid ferrochelatase two mutant

Fig. 2

ATG8a does not associate with 1O2-stressed chloroplasts in fc2 seedlings. The localization of GFP-ATG8a was assessed in stressed fc2-1 seedlings. Shown are representative images of GFP-ATG8a in four day-old fc2-1 seedlings grown in 24 h constant light or 6 h light/18 h dark cycling light. The same line was also subjected to dark-induced carbon starvation for six additional days. Also shown are representative images of untransformed fc2-1 plants from each condition. Shown are the GFP signal (green) and merged overlays with chlorophyll autofluorescence (red). White arrows indicate GFP-ATG8a punctae in cells. Boxes with dashed lines mark magnified sections included on bottom right of select panels. Scale bars = 10 µm

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