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Table 5 Summary of candidate genes for detected QTL in apple genome GDDH13 and result of the blast to genome of other pear species

From: Genome-wide association study of individual sugar content in fruit of Japanese pear (Pyrus spp.)

     

BLAST to genome of European pear (P. communis L. ‘Bartlett’)

BLAST to genome of wild pear in China (P. betulaefolia)

SNP

Trait

Candidate gene in GDDH13

ID

Position of candidate gene in GDDH13

Position 

Identity (%)

Bit score

Position

Identity (%)

Bit score

Chr07_33139082

SUC,FRU,GLC

Acid invertase, MdoVIN2

MDP0000377084

Chr7:32184887

Chr7:23975117

93.9

1893

Chr7:24394889

94.0

1892

 

SUC

Sucrose synthase 4

MD15G1223500

Chr15:18156590

Chr15:14168216

96.3

3561

Chr15:14346485

96.2

3544

 

FRU

Sucrose synthase 4

MD15G1223500

Chr15:18156590

Chr15:14346485

96.3

3561

Chr15:14346485

96.2

3544

Chr04_29871378

FRU

ERD6-like gene

MD04G1211000

Chr4:29523287

Chr4:20641984

93.7

1404

Chr4:25696155

95.1

1467

Chr11_41197041

FRU, GLC

A tandem repeat of ERD6-like genes

MD11G1293100

Chr11:41256007

Chr11:28621080

95.4

4401

Chr11:33736771

95.8

4455

  

A tandem repeat of ERD6-like genes

MD11G1293200

Chr11:41260335

Chr11:28626231

95.7

5312

Chr11:33741882

95.2

5230

  

A tandem repeat of ERD6-like genes

MD11G1293300

Chr11:41264834

Chr11:28631364

94.9

616

Chr11:33746689

94.9

614

  

A tandem repeat of ERD6-like genes

MD11G1293400

Chr11:41270166

Chr11:28638653

95.4

5738

Chr11:33757627

95.9

3607

Chr03_31587739

HarT

NAC18.1 transcription factor

MD03G1222600

Chr3:30696191

Chr3:18264874

96.6

1616

Chr3:25257554

96.8

1626

Chr15_16568005

HarT

ACS gene family

MD15G1203500

Chr15:16180415

Chr15:12799631

96.9

1882

Chr15:12917853

95.0

1773

Chr16_3043313

Aci

Aluminum-activated malate transporter-like gene, ma1

MDP0000252114

Chr16:3176495

Chr16:2447780

95.9

2486

Chr16:2478566

96.1

2512

  1. Traits are as defined in Table 2
  2. MDP0000377084 and MDP0000252114 are IDs from the Malus × domestica genome v1.0, which is the previous version of GDDH13
  3. The sequences of candidate genes in GDDH13 were compared to the genome sequences of P. communis L. ‘Bartlett’ and P. betuleafolia using a local installation of blastn 2.7.1
  4. SUC = sucrose content, FRU = fructose content, GLC = glucose content, SOR = sorbitol content, HarT = harvest time, Aci = acidityÂ