Skip to main content
Fig. 1 | BMC Plant Biology

Fig. 1

From: Development of a Csy4-processed guide RNA delivery system with soybean-infecting virus ALSV for genome editing

Fig. 1

Development of CCAC. (A) Stop codon analysis of gRNA. (N)20denotes the 20 bp spacer sequence. Stop codons in frame 1 are highlighted in red, in frame 2 highlighted in yellow, and in frame 3 highlighted in blue. (B) Schematic representation of ALSV-RNA2 vector. The multiple cloning site (MCS)XhoI-XmaI-BamHI is located between the artificial Q/G protease cleavage sites. The BsaI sites were introduced immediately downstream of the translation stop codon of the Vp24 protein of 108k polyprotein, following the C4 site. attL1 and attL2 sequences are flanking the ALSV-RNA2 expression cassette. BsaI sites are highlighted in yellow, while their recognition sites are also underlined. C4 site is highlighted in blue. The red arrows represent the various cleavage sites

Back to article page