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Fig. 2 | BMC Plant Biology

Fig. 2

From: Development of a Csy4-processed guide RNA delivery system with soybean-infecting virus ALSV for genome editing

Fig. 2

Infectivity of CCAC. (A) Diagram of CCAC:vPDS + gPDS. The gPDS (gRNA targeting PDS gene) flanked by two C4 sites, cloned downstream of 108k polyprotein. A 102 bp fragment of PDS (shown as “vPDS”) inserted into MCS, to silence PDS gene by VIGS. (B) Plants were agroinoculated with empty vector (Mock), p301ALR1 + p301ALR2(CCAC), p301ALR1 + p301ALR2-vPDS (CCAC:vPDS) or p301ALR1 + p301ALR2- vPDS -gPDS (CCAC:vPDS + gPDS). The photobleaching of leaves was observed in CCAC:vPDS and CCAC:vPDS + gPDS. Photos were taken 3 weeks post-inoculation. (C) Analysis of mRNAs in virus infected plants by RT-PCR. Total RNA was extracted from plants inoculated by Mock or CCAC:vPDS+gPDS (v+g). For “v+g”, the leaves of which one third of the surface area had been photobleached were used for RNA extraction. The housekeeping gene EF1a, and ALSV-RNA1 expression were used as the control. The positions of primers F1, R1, R2, F3 and R3 to detect ALSV-RNA2 were shown in A. Uncropped full-length gels of Figure 2C is shown in Supplementary Figure 8

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