Fig. 1

Changes in size class and 5′ nucleotide of B. napus sRNAs in response to Sclerotinia sclerotiorum infection. AA principal component analysis based on normalized read counts from DESeq2. The x axis shows principal component 1, which explained 99% of the variance, and the y axis shows principal component 2, which explained 1% of the variance. The infected samples are depicted with red circles and the mock samples depicted in turquoise. B Histogram of read sizes from pooled mock and infected samples. Inset: a representative picture of B. napus leaves under both treatments (24 h post-inoculation (HPI)). Left: for the pooled replicates of the mock sample, y axis depicts the percentage of reads across all three replicates and the x axis read length in nucleotides. Right: for the pooled replicates of the infected sample, same information as for the mock sample. C Results from pooled replicates for the mock (left) and infected (right). Showing percentage of reads (y axis) of each size class (x axis) that had each of the four nucleotides (AGCU) in their 5′ position