Fig. 5

Degradome validation of a TAS gene triggered by miRNA 1885. A Target plot (T-plot) of the cleavage site on a TAS gene putatively targeted by miRNA 1885. The transcript position in the target gene is on the x-axis and the y-axis shows the 5′ read coverage at each position; the cleavage site predicted by PARESnip2 is labelled in red. The category and p value of the PARESnip2 test are given in the top left hand corner of the graph. B T-plot of cleavage site on a galactose oxidase gene putatively targeted by one of the ta-siRNAs produced by the miRNA 1885-triggered TAS gene. The x-axis shows the transcript position in the target gene and the y-axis shows read 5′ coverage at each position; the cleavage site predicted by PARESnip2 is labelled in red. The category and p value from PARESnip2 are given in the top right hand corner of the graph. C A heat map of 9 differentially expressed PHAS loci plotted with normalized counts from DESEq2