Fig. 6

5′ Rapid amplification of cDNA ends (RACE), degradome result, and qPCR for an ethylene response factor gene putatively cleaved by a novel sRNA. A Gel electrophoresis of the 5′-RACE result showing a band of the correct size; the second lane is a no template control. The full gel image is in Supplementary Fig. 1. B Sequence complementarity of the sRNA and its target. The blue arrow shows the cleavage site identified from sequencing the 5′-RACE product and the red arrow shows the cleavage site identified with degradome sequencing. C Target-plot (T-plot) of the degradome result of the 5′-RACE validated gene showing the transcript position in the target gene (x-axis) and read 5′ coverage at each position (y-axis); the cleavage site predicted by PARESnip2 is labelled in red. D RT-qPCR result of target gene in mock and infected sample (x-axis) and relative expression of gene to the house keeping actin gene