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Table 1 An overall summary of the sequencing data

From: Identification of Brassica napus small RNAs responsive to infection by a necrotrophic pathogen

Sample

Raw reads

Clean filtered reads*

Unique B. napus reads†

Structural RNA‡

Clean mappable reads††

Uniquely mapped reads

Reads mapping to multiple locations

Total mapped to B. napus

Total mapped to B. napus (%)

Mock

23,754,736

20,144,362

8,944,885

318,393

8,626,492

1,047,207

7,416,613

8,463,820

98.1

Mock

22,377,118

18,983,632

7,767,860

311,538

7,456,322

1,103,293

6,242,937

7,346,230

98.5

Mock

31,509,749

26,430,696

11,421,524

450,137

10,971,387

1,535,228

9,301,292

10,836,520

98.8

Infected

26,483,807

20,626,924

3,779,014

202,125

3,576,889

445,577

2,310,074

2,755,651

77

Infected

23,182,766

18,818,235

3,866,998

223,567

3,643,431

462,630

2,214,409

2,677,039

73.5

Infected

24,782,597

21,884,135

6,016,997

508,508

5,508,489

570,565

4,180,632

4,751,197

86.3

Total

152,090,773

126,887,984

41,797,278

2,014,268

39,783,010

5,164,500

31,665,957

36,830,457

87.3

  1. *Reads after size and adapter filtering
  2. †Reads that mapped best to the B. napus genome from Bbsplit
  3. ‡Reads that potentially originated from structural RNAs (ribosomal RNA, snRNAs, e.t.c)
  4. ††Reads after removing potential structural RNAs that were used for ShortStack