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Table 3 An overview of the characteristics of PHAS loci identified using PHAS tank

From: Identification of Brassica napus small RNAs responsive to infection by a necrotrophic pathogen

PHAS locus ID

Length

Phased ratio

Phased abundance

Phased number

Phased score

Triggering miRNAa

Description of BLAST hit

Differential expression

chrCnn_random_466

791

0.398

3845

16

52.538

 

Uncharacterized

 

chrA04_130

917

0.448

274

19

47.821

miR4995, miR6035

chloroplast

 

chrC01_614

331

0.568

3417

7

32.332

 

Uncharacterized

Upregulated

chrA03_random_16

434

0.6

630

8

30.96

 

Disease resistance protein

Upregulated

chrA01_443

245

0.79

877

5

26.769

miR390

non coding RNA

Downregulated

chrUnn_random_22

621

0.468

159

11

26.078

 

chloroplast

 

chrC09_754

621

0.468

159

11

26.078

 

chloroplast

 

chrA01_random_47

245

0.769

877

5

26.05

 

non coding RNA

Downregulated

chrA07_478

287

0.621

823

6

25.004

 

metal tolerance protein

 

chrA01_532

371

0.666

111

7

21.953

 

Disease resistance protein

Upregulated

chrC09_1169

392

0.747

1217

4

21.216

miR838

Uncharacterized

 

chrA02_413

455

0.381

2189

7

20.533

 

Uncharacterized

 

chrAnn_random_753

308

0.531

547

6

20.073

miR390

non coding RNA

 

chrA09_223

476

0.314

579

10

19.96

miR1885

Disease resistance proteinb

 

chrC05_811

392

0.393

559

8

19.894

miR838

non coding RNA

 

chrA01_533

329

0.746

288

4

16.888

 

Disease resistance protein

Upregulated

chrC02_807

371

0.668

109

5

15.662

 

Disease resistance protein

 

chrAnn_random_716

413

0.7

182

4

14.562

 

Uncharacterized

 

chrA10_253

329

0.376

198

7

13.935

 

Photosystem II protein D1

Upregulated

chrC01_385

266

0.444

237

5

12.145

 

non coding RNA

 

chrC09_1045

266

0.4

152

5

10.041

 

COP9 signalosome complex subunit

Downregulated

chrC09_1044

434

0.373

788

4

9.949

 

Pentatricopeptide repeat

 

chrCnn_random_695

391

0.35

234

5

9.558

 

Uncharacterized

 

chrA04_52

413

0.359

206

5

9.558

 

Uncharacterized

 

chrC08_352

392

0.46

118

4

8.772

 

Uncharacterized

Downregulated

chrC07_411

350

0.305

175

4

6.294

 

Uncharacterized

 
  1. aBased on psRNA target comparison of conserved miRNA sequences with all PHAS loci
  2. bEvidence for cleavage from degradome sequencing