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Fig. 2 | BMC Plant Biology

Fig. 2

From: Pathogen and drought stress affect cell wall and phytohormone signaling to shape host responses in a sorghum COMT bmr12 mutant

Fig. 2

Experimental design of pathogen inoculation and watering treatments. A. The experimental design was a randomized split block by watering conditions with eight replicates over time and watered with a fertilizer-water mixture. RNA sequencing was performed on samples representing all water and inoculum treatments for all 3 days after inoculation (DAI), and performed on samples with PDB or Fusarium thapsinum and both water treatments for bmr12 and the wild-type at 0 and 13 DAI, as indicated. Phytohormones and phenolic metabolites were analyzed from samples collected at 3 DAI from wild-type and bmr12 plants with mock or F. thapsinum samples for both water treatments. B. Sample collection from plants with lesion lengths < 20 mm: A 2-cm section around the inoculation site was used for RNA sequencing (“A” section). Then, 1 cm distal to this section, a separate 2-cm tissue section was analyzed for phenolic metabolites and phytohormones (“C” section). C. Sample collection from plants with lesion lengths > 20 mm: A 2-cm section from the base of the lesion was used for RNA sequencing (“A” section). A 2-cm sample immediately adjacent to the base of the lesion (“B” section) was collected but not used in this study. Then, 1 cm distal to this section, a separate 2-cm tissue section was analyzed for phenolic metabolites and phytohormones (“C” section)

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