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Fig. 7 | BMC Plant Biology

Fig. 7

From: Regulation of wound ethylene biosynthesis by NAC transcription factors in kiwifruit

Fig. 7

Promoter activation by NAC (NAM, ATAF1/2, CUC2) and EIL (Ethylene-insensitive3-like) TFs using deletions of the A. chinensis ‘Hort16A’AcACS1 promoter. Different sized AcACS1 regulatory regions (< 1000 bp) upstream of the ATG (AcACS1pro + length in bp) were cloned upstream of the LUC reporter gene of pGreenII-0800LUC in frame with the start ATG and tested for transient activation in N. benthamiana. LUC/REN luminescence ratio values of transcription factors AcNAC1-AcNAC4 and a pool of AcEIL1–4 (equal mixture of AcEIL1–4) were compared to a GUS control construct which was set to 1. Statistical differences were determined by Tukey’s honest significant difference test (HSD) after analysis of variance (ANOVA) analysis compared to GUS. Data are mean ± SE, n = three biological replicates (plants), * different at p < 0.05

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