Fig. 7
From: Enhancement of plant cold tolerance by soybean RCC1 family gene GmTCF1a
Increase in cold tolerance by overexpression of GmTCF1a in Arabidopsis. A The expression level of GmTCF1a in two 35S:GmTCF1a transgenic plants. The UBC gene was used as a control. B The survival rate of wild-type and 35S:GmTCF1a lines after freezing treatment from b. C Freezing assay of wild-type and two transgenic Arabidopsis lines (#14–4 and #17–3) at − 8 °C for 2.5 h after cold acclimation. Photographs were taken 7 days after freezing treatment. D Electrolyte leakage from wild-type and two 35S:GmTCF1a lines after being treated at temperatures of 0, − 2, − 4, − 6, and − 8 °C for 0.5 h. Error bars represent the Mean ± SD from three technical replicates. One-way ANOVA with a Tukey test was performed to explore the significant differences at − 6 and − 8 °C treatment, and * indicates P < 0.05. These experiments were repeated three times and obtained the same results