Fig. 7From: Identification of C3H2C3-type RING E3 ubiquitin ligase in grapevine and characterization of drought resistance function of VyRCHC114E3 ubiquitin ligase activity of VyRCHC114. a Determination of E3 ubiquitin ligase activity of VyRCHC114; an immunoblot analysis was performed with the ubiquitin antibody (right) and MBP antibody (left). b Determination of E3 ubiquitin ligase activity of VyRCHC114 mutants; an immunoblot analysis was performed with ubiquitin antibody (right) and MBP antibody (left). c Schematic diagram of VyRCHC114 C3H2C3 domain and putative mutation sites. C328S and N355A affect a non-conserved site of the VyRCHC114 C3H2C3 domain. Mutations in C320S and H341A affect the ubiquitin activity of VyRCHC114Back to article page