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Fig. 4 | BMC Plant Biology

Fig. 4

From: LncRNA TCONS_00021861 is functionally associated with drought tolerance in rice (Oryza sativa L.) via competing endogenous RNA regulation

Fig. 4

Verification of competitive interaction between TCONS_00021861/miR528-3p/YUCCA7. a: The expression levels of TCONS_00021861, YUCCA7 and miR528-3p were examined by RT-qPCR in drought stressed vs. control. b: Expression correlation between TCONS_00021861/miR528-3p/YUCCA7 in stressed leaf tissues. c: Potential miR528-3p cleavage sites on TCONS_00021861 and YUCCA7 identified by RLM-5′ RACE. d: YUCCA7 expression in response to overexpression of TCONS_00021861 and miR528-3p. e: IAA content of mesophyll cells determined by HPLC. f: Pearson correlation coefficients between TCONS_00021861/miR528-3p/YUCCA7. EV: empty vector; LNC: TCONS_00021861 overexpression; MIR: miR528-3p overexpression; LNC + MIR: co-overexpression of TCONS_00021861 and miR528-3p; C: the control without any treatment; D: treatment by 20 % PEG 6000; *P < 0.05, **P < 0.01, ***P < 0.001

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