Fig. 6
The phenotypes of overexpression lines of LsaGRF5 and Lsa-miR396a in lettuce. A Verification of positive overexpression lines of LsaGRF5-OE and Lsa-miR396a-OE. The samples used double-distilled H2O (ddH2O) and transformed vector as template were designated as negative and positive control, respectively. B Expression assay of LsaGRF5 and Lsa-miR396a in LsaGRF5-OE and Lsa-miR396a-OE lines. The expression level of one of three biological replications in negative control was defined as “1”. Values represent means ± SD (n = 3) from three biological replicates. C The phenotype of T1 progeny of LsaGRF5-OE. The seeds of T1 progeny of LsaGRF5 overexpression line and empty vector transgenic line (OE-) were screened on the plates containing Kanamycin. OE- was used as a negative control. D The phenotype of T1 progeny of Lsa-miR396a-OE. The seeds of T1 progeny of Lsa-miR396a overexpression line and wild type lettuce ‘YDL’ were sowed and planted in greenhouse at the same time. ‘YDL’ was used as a negative control. e. Leaf length and width of LsaGRF5, Lsa-miR396a overexpression lines and their controls at the 10-leaf stage. The outermost leaves were subjected to measurements. Box plots display median (line), interquartile range (box), and whiskers (extending 1.5 times the interquartile range). n > 30. The statistical significances were determined using Student’s t test. ***P < 0.001